CARRIER FREQUENCY OF AUTOSOMAL RECESSIVE DISORDERS (BC, BLAD, FXID AND CVM) IN HOLSTEIN COWS IN JALISCO, MEXICO

Objective: This study examined the carrier frequency of bovine citrullinemia (BC), bovine leukocyte adhesion deficiency (BLAD), factor XI deficiency (FXID), and complex vertebral malformation (CVM) autosomal recessive disorders in Bos taurus Holstein cows bred in the Altos Norte region of the state of Jalisco, Mexico. Methods: We extracted DNA from 408 random samples of peripheral blood, and then used polymerase chain reaction to identify insertion mutations for FXID, and polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) for CVM, BC and BLAD. We visualized the PCR products using agarose gel electrophoresis stained with GelRed®. Results: We found that 100% of wild-type (N/N) allele homozygous animals for genes CD18, ASS, and FXI were free of the mutations for BLAD, BC and FXID respectively. For gene SLC35A3 we estimated total carrier frequency of 10.3% and allele frequency of 5%. Conclusion: We found no evidence of mutant alleles for the recessive hereditary disorders BC, BLAD, and FXID. For the SLC35A3 mutation associated with the hereditary CVM disorder, we observed some carriers animals despite the efforts of other countries to counteract the impact of these harmful mutations.