SOME TRITERPENIC COMPOUNDS IN EXTRACTS OF CECROPIA AND BAUHINIASPECIES FOR DIFFERENT SAMPLING YEARS

The aim of this paper is to provide an overview on the chemical composition of triterpenes in widespread used folk medicine species, through the development and validation of 11 compounds using high performance liquid chromatography with UV detection (HPLC-UV). The compounds were separated using isocratic elution, on a reverse phase column (Kinetex C18, 250 mm × 4.6 mm, 5μm) with mobile phase consisted of acetonitrile: tetrahydrofuran (90:10, v/v), flow-rate of 0.5 ml/min and detection in 210 nm. Diverse validation parameters were successfully evaluated. The samples of Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Cecropia palmata and Cecropia obtusa collected in 2012, 2013 and 2014 from Amazon were treated with two different solvents (ethyl acetate and chloroform) and analyzed by the proposed method. Stigmasterol, lupeol, β-sitosterol, β-amirin and α- amirin were found in all the studied plants. Hightlingting the presence of oleanolic acid, maslinic acid in Cecropia obtusa and Cecropia palmata extracts, erythrodiol only in Cecropia palmata, stigmasteol in Bauhinia variegata var. variegata and α-amirin in Bauhinia variegata var. alboflava. Overall, ethyl acetate showed better performance as the extractor solvent than chloroform. Moreover, it could be used for the quality control of medicinal plants and to assess potential marker compounds.