POSSIBLE NEW DISCOVERIES IN BIFIDOBACTERIUM BREVE ANNOTATION THROUGH BIOINFORMATICS ANALYSIS

Objectives: The increase in genomes deposited in databases has impacted the increases in hypothetical proteins deposited. Functional identification of these proteins can lead to a better understanding of the biological processes and molecular functions that they can perform. In addition, functional annotation can also lead to discoveries in target organisms of interest, such as Bifidobacterium breve, which is described as one of the components of the human microbiota and is functionally involved in maintenance of health. Methods: This work aimed to perform the functional annotation of hypothetical proteins from the genome of Bifidobacterium breve strain DSM 20213 = JCM 1192. The reference genome used is available in the public database NCBI, under accession number NZ_AP012324.1. The hypothetical proteins were extracted using the Artemis software. For functional annotation, the following were used: CDART, SMART, CD-Search tool and ProtoNet software. Results: A total of 273 hypothetical protein sequences were analyzed, among which, 28 presented significant results, and of these we can mention the BBBR_RS05180 sequence, which is involved in the direction of cells during hematopoiesis. And the BBBR_RS06455 sequence that has the molecular function of selectively and not covalently interacting with peptidoglycan. Conclusion: The characterization of hypothetical proteins may be related to a better understanding of several biological factors such as metabolic pathways, drug development, disease control strategies, among others.