LARVAL DEVELOPMENT AND PROTEOLYTIC ACTIVITY OF ANTICARSIA GEMMATALIS HÜBNER (LEPIDOPTERA: NOCTUIDAE) EXPOSED TO DIFFERENT SOYBEAN PROTEASE INHIBITORS
Neilier Rodrigues da Silva-Junior
Silva-Junior, N. R.
Eduardo Gomes de Mendonça
Mendonça, Eduardo Gomes de
Rafael de Almeida Barros
Barros, Rafael de Almeida
Gláucia Cordeiro
Cordeiro, Gláucia
Carolina R. da Silva
Silva, Carolina R. da
Welligton G. Campos
Campos, Welligton G.
Joel A. de Oliveira
Oliveira, Joel A. de
Maria Goreti de Almeida Oliveira
Oliveira, M. G. A.
01/05/2022
477-489
42
Anticarsia gemmatalis represents a relevant factor for lowering soybean and other legume crop productivities. Protease inhibitors affect protein degradation and reduce the availability of amino acids, impairing the development and survival of insect pests. To evaluate the possible use of proteinaceous protease inhibitors in the management of this pest, the activities of midgut proteases and the growth and development of A. gemmatalis larvae exposed to soybean Bowman-Birk trypsin‐chymotrypsin inhibitor (SBBI) and soybean Kunitz trypsin inhibitor (SKTI) were determined. The survival curves obtained using Kaplan-Meier estimators indicated that SKTI and SBBI stimulated larval survival. However, the development of A. gemmatalis was delayed, and prepupal weight decreased in the presence of both inhibitors. The results showed that SKTI and SBBI inhibited the trypsin‐like and total proteolytic activities of larvae on the 12th day after eclosion. On the 15th day after eclosion, larvae exposed to SKTI increased the activities of trypsin and total proteases. Although SKTI and SBBI did not affect the survival of the insect, they had effects on midgut proteases in a stage where in A. gemmatalis fed voraciously, increased the larval cycle, and decreased prepupal weight. These findings provide baseline information about the potential of proteinaceous protease inhibitors to manage the velvetbean caterpillar, avoiding chemical pesticides.
Biotic stress, Insect attack, Insect performance, Protease inhibitory.
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