ESSENTIAL OIL FROM EUGENIA PYRIFORMIS CAMBESS AERIAL PARTS AND ITS MAJOR CONSTITUENT LIMONENE AS AN AGENT WITH ANTI-ACETYLCHOLINESTERASE AND ANTICARIOGENIC ACTIVITIES
Cassia Cristina Fernandes
Fernandes, C. C.
Mariana Brentini Santiago
Santiago, M. B.
Carlos Henrique Gomes Martins
Martins, C. H. G.
Jackson Breno Amaral Silva
Silva, J. B. A.
Antônio Eduardo Miller Crotti
Crotti, A. E. M.
Mayker Lazaro Dantas Miranda
Miranda, M. L. D.
31/08/2023
510-522
43
This study aimed at determining, for the first time, anticariogenic and anti-acetylcholinesterase activities of essential oil from Eugenia pyriformis aerial parts (EP-EO) and its major constituent limonene against cariogenic bacteria and the enzyme acetylcholinesterase (AChE). EP-EO and limonene were subject to the broth microdilution method on 96-well microplates and determination of minimum inhibitory concentration (MIC) and minimum inhibitory concentration of biofilm (MICB50) (µg/mL). AChE inhibition was qualitatively evaluated by Thin Layer Chromatography (TLC). EP-EO and limonene not only showed strong anticariogenic activity, since their MIC and MICB50 values ranged between 20 and 400 µg/mL, but also inhibited cholinesterase when subject to TLC. Both GC-MS and GC-FID analyses identified and quantified the following major constituents: limonene (14.8%), nerolidol (11.0%) and α-cadinol (10.3%). This study showed that EP-EO and limonene were active in in vitro assays and, thus, may be important targets of further in vivo studies.
tooth decay, Alzheimer’s disease, acetylcholinesterase, Streptococcus mutans, limonene.
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